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Easier detection of invertebrate "identification-key characters" with light of different wavelengths ArchiMer
Koken, Marcel; Grall, Jacques.
The marine alpha-taxonomist often encounters two problems. Firstly, the "environmental dirt" that is frequently present on the specimens and secondly the difficulty in distinguishing key-features due to the uniform colours which fixed animals often adopt. Here we show that illuminating animals with deep-blue or ultraviolet light instead of the normal white-light abrogates both difficulties; dirt disappears and important details become clearly visible. This light regime has also two other advantages. It allows easy detection of very small, normally invisible, animals (0.1 mu m range). And as these light wavelengths can induce fluorescence, new identification markers may be discovered by this approach.
Tipo: Text Palavras-chave: Fluorescent colours; Light emission; Determination; Cryptic species; Dimorphism; Pattern UV light; Deep-blue light; Autofluorescence.
Ano: 2011 URL: http://archimer.ifremer.fr/doc/00185/29660/28026.pdf
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What are tilosomes? An update and new perspectives Acta Botanica
Kedrovski,Halisson Rafael; Sajo,Maria das Graças.
ABSTRACT Characterization of tilosomes and study of their development revealed that they are similar to the cell wall ingrowths of transfer cells. Roots from Anathallis sclerophylla were used to examine the development, histochemistry and ultrastructure of tilosomes using light, confocal and electron microscopy (both SEM and TEM). Tilosomes initiate as cellulosic papillae in the root elongation zone, increasing the apo/symplast surface throughout the wall-membrane apparatus. Both the development and structure of tilosomes and velamen strips appear similar to the reticulated and flange cell wall ingrowths of Vicia faba transfer cells. We suggest two functional phases for tilosomes: a) in young tissues they increase the symplast connection and thus improve...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Autofluorescence; Callose; Cell wall biochemistry; Ontogeny; Oriented wall deposition; Waxes.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33062019000100106
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